RESOURCES

TEACHER CONFERENCE 2008

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Are you a creative teacher who experiments with science? Then plan to attend the 2008 Michael Smith Labs Teacher Conference. During this unique conference, you will: 1) interact with scientists, 2) participate in fun science literacy activities, 3) collaborate with other teachers, and 4) build your own interactive science activities for the classroom.

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Amplification of a specific DNA sequence by Polymerase Chain Reaction (PCR)

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In this experiment you would use DNA isolated from your cheek cells (see above) as template to amplify a short region of DNA within the plasmogen activator gene (TPA) containing a short 300bp insertion called an Alu element. Alu elements are short interspersed, repeated DNA elements distributed through out the primate genomes. The Alu element found within an intron of the TPA gene is dimorphic, i.e. it is present in some individuals but not in others. Following PCR amplification and electrophoresis you would be able to determine if the individual is heterozygous or homozygous for the presence or absence of the Alu insertion.

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