Previewing of videos: In the past, I would have given these lectures in class, but our feedback suggested that the amount of lecturing (along with the practical exercises) can be overwhelming. Furthermore, due to timing, many of the in person lectures would occur after the experiment had already started. Consequently, we’ve been using a hybrid model, where lectures have been pre-recorded so that you can watch ahead of time. For best pedagogy, please watch these videos in the order and with the schedule of lab activities in mind (see calendar). Note that lecture content gets less intense as the week progresses.
Before Monday, June 19th:
Replication_01 (52min) | PCR_video_01 (37min) | PCR_video-02 (89min)
Before Tuesday, June 20th:
cloning_video_01 (56min) | cloning_video-02 (62min) | NGS01_video_01 (25min)
Before Wednesday, June 21st:
RNA_video_01 (47min) | RNA_video_02 (32min) | NGS02_video (51min)
Before Thursday, June 22nd:
plasmid_prep_video_01 (25min) | SDS-PAGE_video_01 (30min)
Before Friday, June 23rd:
Western_video_01 (35min)
Next Gen Sequencing data link: (will provide when we’ve done the experiment)